Proinsulin EIA 胰岛素原检测试剂盒

Intended use

Proinsulin EIA 胰岛素原检测试剂盒Mercodia Proinsulin ELISA provides a method for the quantitative determination ofhuman proinsulin in serum or plasma.

Summary and explanation of the test

Proinsulin EIA 胰岛素原检测试剂盒Proinsulin is the precursor of insulin which is the principal hormone responsiblefor the control of glucose metabolism. It is synthesized in the ß-cells of the Isletsof Langerhans and is subsequently processed to form C-peptide and insulin.High proinsulin concentrations are usually noted in patients with benign ormalignant ß-cell tumors of the pancreas. Most patients with ß-cell tumors haveincreased insulin, C-peptide and proinsulin concentrations, but occasionallyonly proinsulin is elevated. Despite its low biological activity, proinsulin maybe increased sufficiently to produce hypoglycemia. Increased proinsulinconcentrations may also be detected in patients with renal failure, cirrhosis orhyperthyroidism.

Principle of the procedure

Proinsulin EIA 胰岛素原检测试剂盒Mercodia Proinsulin ELISA is a solid phase two-site enzyme immunoassay.It is based on the sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the proinsulin molecule.During incubation, proinsulin in the sample reacts with anti-proinsulin antibodiesbound to microtitration well. After washing, peroxidase-conjugated anti-proinsulinantibodies are added and after the second incubation and a simple washing stepthat removes unbound enzyme labeled antibody, the bound conjugate is detectedby reaction with 3,3’,5,5’-tetramethylbenzidine (TMB). The reaction is stopped byadding acid to give a colorimetric endpoint that is read spectrophotometrically