Iso-Insulin EIA 同工胰岛素检测试剂盒介绍：

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of insulin in human serum or plasma.

Iso-Insulin EIA 同工胰岛素检测试剂盒Summary and explanation of the test

Insulin is the principal hormone responsible for the control of glucose metabolism.It is synthezised in the ß-cells of the islets of Langerhans as the precursor,proinsulin, which is processed to form C-peptide and insulin. Both are secretedin equimolar amounts into the portal circulation. The mature insulin moleculecomprises two polypeptide chains, the A chain and B chain (21 and 30 aminoacids respectively). The two chains are linked together by two inter-chaindisulphide bridges. There is also an intra-chain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration,and the hormone has a number of important metabolic actions. Its principalfunction is to control the uptake and utilization of glucose in peripheral tissuesvia the glucose transporter. This and other hypoglycaemic activities, such as theinhibition of hepatic gluconeogenesis and glycogenolysis are counteracted by thehyperglycaemic hormones including glucagon, epinephrine (adrenaline), growthhormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetesmellitus (IDDM) and some other conditions such as hypopituitarism. Insulinlevels are raised in non-insulin-dependent diabetes mellitus (NIDDM), obesity,insulinoma and some endocrine dysfunctions such as Cushing’s syndrome andacromegaly.

Iso-Insulin EIA 同工胰岛素检测试剂盒Principle of the procedure

Mercodia Iso-Insulin ELISA is a solid phase two-site enzyme immunoassay. It isbased on the direct sandwich technique in which two monoclonal antibodies aredirected against separate antigenic determinants on the insulin molecule. Duringincubation insulin in the sample react with peroxidase-conjugated anti-insulinantibodies and anti-insulin antibodies bound to microtitration well. A simplewashing step removes unbound enzyme labeled antibody. The bound conjugate isdetected by reaction with 3,3’,5,5’-tetramethylbenzidine. The reaction is stoppedby adding acid to give a colorimetric endpoint that is read spectrophotometrically